The Conestoga Boys Lacrosse Program consistently ranks among the top programs (public and private) in the state as well as nationally. 

 

Conestoga has played in several PIAA State Championship games, dating back to its start in the spring of 2009. The Pioneers lost to LaSalle High School in the first-ever state title game in 2009. Conestoga went on to win three straight state championships, including a 10–6 win against LaSalle in the 2010 PIAA State Championship and a 13-4 win over St. Joes Prep in 2011. After graduating nine Division I players in 2012, the Pioneers lost to LaSalle College High School 13–4 in the 2013 State Championship game.

 

In March 2011, #2 ranked Conestoga faced off against #1 ranked Haverford School, the first time in the history of Pennsylvania High School lacrosse the #1 and #2 Nationally ranked teams met on field. On March 22, 2012, #3 Conestoga defeated #1 Calvert Hall High School (MD) 11–10 at Calvert Hall. The Pioneers then became the top-ranked team in the nation after that game, which they held until losing 12–11 to Garden City. Conestoga has former players who have gone on to play for college powerhouses such as Syracuse, UVA, Duke, Loyola, Maryland, Cornell, University of Notre Dame, Georgetown, Ohio State, Delaware and Drexel.

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On March 24, 2017, Conestoga boys lacrosse team took down Calvert Hall College (Md.) 13-12 (OT) who were ranked #9 nationally at the time. The Pioneers went on to win the District 1 final for the first time in 5 years. The Pioneers lost to Avon Grove High School in the PIAA State Championship in 2017 in a double OT thriller.